ABSTRACT
Sonoclot analyzer has been widely used in many countries. But the reference intervals provided by the manufacturer were derived from only 45 participants, and there was no cut-off value for transfusion for Sonoclot analysis. This study aimed to establish reference intervals and transfusion criterion for Sonoclot analysis. Volunteers were recruited from healthy Chinese adults and patients undergoing cardiac surgery. Blood samples were withdrawn from forearm vein and measured for activated clotting time (ACT), clot rate (CR), platelet function (PF), activated partial thromboplastin time (APTT), fibrinogen concentration (FIB), and platelet count (PLT). The reference intervals were determined by the nonparametric method. Cut-off values were determined by the receiver operating characteristics curve. A total of 135 healthy volunteers and 281 patients were enrolled. The 95% reference intervals were 96-195 s, 22-51 signal U/min, >1.6 for ACT, CR, PF respectively. In the 281 patients, the results of APTT, FIB, PLT, ACT, CR, and PF ranged from 20.5-300.0 s, 0.28-4.11 g/L, (19.0-387.3)×109/L, 80-514 s, 2.9-74 signal U/min, and 0.1-5.1 respectively. The cut-off values for transfusion were >208, ≤14, and ≤1.3 for ACT, CR, PF respectively. The cut-off values of Sonoclot analysis were within the manufacturer's reference intervals, while they were outside the reference intervals established in this study. The results suggested that the manufacturer's reference intervals were not suitable for Chinese. The reference intervals and cut-off values established in this study will be helpful to Chinese patients.
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Blood Coagulation , Cardiopulmonary Bypass , China , Fibrinogen , Metabolism , Partial Thromboplastin Time , Methods , Platelet Count , Point-of-Care Systems , Reference ValuesABSTRACT
<p><b>OBJECTIVE</b>To study the effect of Jinguo Weikang Capsule [see text] on the gene expression of H-ras, epidermal growth factor receptor (EGFR), P53 and C-myc of the gastric mucosa in rats with gastric precancerous lesions, and to investigate the action mechanism of JWC on gastric precancerous lesions.</p><p><b>METHODS</b>A rat model with paratypical proliferation of the gastric epithelium mucosa was established by using 60Co irradiation. Rats were divided into the normal group, model group, high-, medium-, low-dose JWC treatment groups, and the vitacoenzyme control group, and were treated for 30 days. The expression of H-ras, EGFR, P53 and C-myc genes of the gastric mucosa was detected by using immunohistochemical methods.</p><p><b>RESULTS</b>The expression and over-expression rates of H-ras, EGFR, P53 and C-myc gene in the high-and medium-dose JWC treatment groups were significantly lower (P<0.05) as compared with those of the model group.</p><p><b>CONCLUSION</b>JWC can inhibit the expression of the H-ras, EGFR, P53 and C-myc genes expression of the gastric mucosa in rats, which may be one of mechanisms involved in suppressing or reversing gastric carcinogenesis.</p>
Subject(s)
Animals , Rats , Drugs, Chinese Herbal , Pharmacology , Gastric Mucosa , Metabolism , Pathology , Gene Expression Regulation, Neoplastic , Immunohistochemistry , Oncogene Proteins , Metabolism , Precancerous Conditions , Metabolism , Pathology , Proto-Oncogene Proteins , Metabolism , Proto-Oncogene Proteins c-myc , Metabolism , Rats, Sprague-Dawley , ErbB Receptors , Metabolism , Tumor Suppressor Protein p53 , Metabolism , ras Proteins , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of coping strategies, social support and personality factors, such as locus of control and type A behavior on occupational stress in nurses.</p><p><b>METHODS</b>Descriptive correlation research method was used in this investigation. One thousand nine hundred and one nurses were included by using instruments of Occupational Stress Instrument, and these nurses were divided into four groups according to the scores of occupational stress.</p><p><b>RESULTS</b>There were significant differences in general health status, coping strategies, social support, type A behavior and locus of control in the 4 groups (P < 0.05). The level of individual occupational stress increased with the fall of the scores of coping strategies, social support and with the elevation of type A behavior, locus of control and general health. Occupational stress had negative relation with coping strategies, social support, and positive relation with personalities and general bad health.</p><p><b>CONCLUSION</b>The level of occupational stress is affected by coping strategies, social support, type A behavior and locus of control.</p>